Rhodamine efflux patterns predict P-glycoprotein substrates in the National Cancer Institute drug screen.

@article{Lee1994RhodamineEP,
  title={Rhodamine efflux patterns predict P-glycoprotein substrates in the National Cancer Institute drug screen.},
  author={Joseph Schweiray Lee and Kenneth Paull and Montserrat Hermos{\'i}n {\'A}lvarez and Curtis D. Hose and Anne P Monks and Michael R Grever and Antonio Tito Fojo and Susan E Bates},
  journal={Molecular pharmacology},
  year={1994},
  volume={46 4},
  pages={
          627-38
        }
}
Fifty-eight cell lines in the National Cancer Institute drug screen were analyzed for their ability to efflux the fluorescent dye rhodamine 123 as a functional assay for P-glycoprotein (Pgp). Using flow cytometry, the rhodamine fluorescence was measured for each cell line under four incubation conditions, i.e., after accumulation in the presence or absence of the Pgp antagonist cyclosporin A and after efflux in rhodamine-free medium in the presence or absence of cyclosporin A. The results in… CONTINUE READING

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