Rho-Kinase–Mediated Contraction of Isolated Stress Fibers

  title={Rho-Kinase–Mediated Contraction of Isolated Stress Fibers},
  author={Kazuo Katoh and Yumiko Kano and Mutsuki Amano and Hirofumi Onishi and Kozo Kaibuchi and Keigi Fujiwara},
  journal={The Journal of Cell Biology},
  pages={569 - 584}
It is widely accepted that actin filaments and the conventional double-headed myosin interact to generate force for many types of nonmuscle cell motility, and that this interaction occurs when the myosin regulatory light chain (MLC) is phosphorylated by MLC kinase (MLCK) together with calmodulin and Ca(2+). However, recent studies indicate that Rho-kinase is also involved in regulating the smooth muscle and nonmuscle cell contractility. We have recently isolated reactivatable stress fibers from… CONTINUE READING