Rhizogenesis in callus from Conference pear (Pyrus communis L.) protoplasts

Abstract

Large numbers (ca 6×106 protoplasts/g f.wt) of viable (80%) protoplasts were isolated from embryo-callus tissues of Conference pear using an enzyme mixture which contained 2.0% (w/v) Meicelase, 2.0% (w/v) Rhozyme HP-150 and 0.03% (w/v) Macerozyme R-10. A medium based on ammonium-free MS salts and supplemented with 2.0 mg/l NAA, 0.5 mg/l BAP and 9% (w/v… (More)
DOI: 10.1007/BF00034453

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Cite this paper

@article{Ochatt2004RhizogenesisIC, title={Rhizogenesis in callus from Conference pear (Pyrus communis L.) protoplasts}, author={Sergio J. Ochatt and J. Brian Power}, journal={Plant Cell, Tissue and Organ Culture}, year={2004}, volume={13}, pages={159-164} }