Rhizobium tropiciteu genes involved in specific uptake of Phaseolus vulgaris bean-exudate compounds

  title={Rhizobium tropiciteu genes involved in specific uptake of Phaseolus vulgaris bean-exudate compounds},
  author={M{\'o}nica Rosenblueth and Michael F. Hynes and Esperanza Mart{\'i}nez-Romero},
  journal={Molecular and General Genetics MGG},
Rhizobium tropici nodulates and fixes nitrogen in bean. In the R. tropici strain CFN299 we identified and characterized teu genes (tropiciexudate uptake) induced by bean root exudates, localized by insertion of a promoter-less Tn5-gusA1 transposon. teu genes are present on a plasmid of around 185 kb that is conserved in all R. tropici strains. Proteins encoded by teu genes show similarity to ABC transporters, specifically to ribose transport proteins. No induction of the teu genes was obtained… 

Diversity of Rhizobium-Phaseolus vulgaris symbiosis: overview and perspectives

Common bean (Phaseolus vulgaris) has become a cosmopolitan crop, but was originally domesticated in the Americas and has been grown in Latin America for several thousand years. Consequently an

Transcriptomic Responses of Rhizobium phaseoli to Root Exudates Reflect Its Capacity to Colonize Maize and Common Bean in an Intercropping System

Genes for the metabolism of sugars and amino acids, flavonoid and phytoalexin tolerance, and a T3SS were expressed in both monocultures and milpa system, which reveals the adaptive capacity of rhizobia to colonize both legumes and cereals.

Rhizobium etli maize populations and their competitiveness for root colonization

Several R. etli types were preferentially encountered as putative maize endophytes, and the dominant and highly competitive strain Ch24-10 was the most tolerant to 6-methoxy-2-benzoxazolinone (MBOA), a maize antimicrobial compound that is inhibitory to some bacteria and fungi.

Genomic basis of symbiovar mimosae in Rhizobium etli

The genomic differences between the two symbiovars, mimosae and phaseoli may explain different host specificity and support that the generalist symbiovar Mimosae may be older than the specialist symb Giovar phaseoli.

Expression Analysis of the Transporters of Sinorhizobium Meliloti

This large scale expression analysis gives insight into the natural environment of S. meliloti by studying those genes that are induced by compounds that would be found in the soil by finding putative transporters for amino acids, sugars, sugar alcohols, amino sugars, betaines and other compounds that might be found on the soil.

Role of plant compounds in the modulation of the conjugative transfer of pRet42a

It is found that bean root exudates or root and nodule extracts induce the CT of pRet42a in the plant rhizosphere, which suggests that the plant could participate at different levels to modulate the CT, and that some compounds could be activating genes in the conjugation machinery.

A Genetic Locus Necessary for Rhamnose Uptake and Catabolism in Rhizobium leguminosarum bv. trifolii

P Phenotypic analyses of representative mutants from this locus provide genetic evidence that the catabolism of rhamnose differs from previously described methyl-pentose catabolic pathways.

Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)

Genome analysis revealed a wide array of traits that may allow these strains to be successful rhizosphere colonizers, including surface polysaccharides, uptake transporters and catabolic enzymes for nutrients, diverse iron-acquisition systems, cell wall-degrading enzymes, type I and IV pili, and novel T1SS and T5SS secreted adhesins.

The Rhizobium leucaenae CFN 299 pSym plasmid contains genes expressed in free life and symbiosis, as well as two replication systems

A functionally diverse group of genes were expressed during discrete stages of the symbiosis or in free-living cells, including genes whose products are involved in nodulation and nitrogen fixation, carbon metabolism, vitamin synthesis, sulfur utilization, conjugation, transposition and DNA replication.

Increased metabolic potential of Rhizobium spp. is associated with bacterial competitiveness.

A statistically significant correlation between the ability to metabolize a broad range of substrates and nodulation competitiveness was found, indicating that metabolic properties may be an essential trait in determining the competitiveness of rhizobia.



Nodulating ability of Rhizobium tropici is conditioned by a plasmid‐encoded citrate synthase

A gene encoding citrate synthase on the pSym plasmid of Rhizobium tropici, a species that forms nitrogen‐fixing nodules on the roots of beans and trees, is found, suggesting a possible role of pcsA in iron uptake.

Isolation and characterization of Rhizobium tropici Nod factor sulfation genes.

Rhizobium tropici produces a mixture of sulfated and non-sulfated Nod factors and the genes responsible for the sulfation process in R. tropici strain CFN299 were cloned and sequenced, and mutants of NodP and NodH were obtained by site-directed mutagenesis.

Increased Bean (Phaseolus vulgaris L.) Nodulation Competitiveness of Genetically Modified Rhizobium Strains

This work has analyzed under laboratory conditions the competitive abilities of the different types of Rhizobium strains capable of nodulating Phaseolus vulgaris L. bean and found that type I R. leguminosarum bv.

The genetic and biochemical basis for nodulation of legumes by rhizobia.

  • S. Pueppke
  • Biology
    Critical reviews in biotechnology
  • 1996
This review focuses on the nodulation genes of rhizobia, with particular emphasis on the concept of biological specificity of symbiosis with legume host plants.

Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov.

A new Rhizobium species that nodulates Phaseolus vulgaris L. nov. is proposed on the basis of a sequence analysis of 16S ribosomal DNA, characterized by the capacity to establish an effective symbiosis with bean plants, the reiteration of the nitrogenase structural genes, and the organization of the common nodulation genes into two separate transcriptional units.

Classification of Austrian rhizobia and the Mexican isolate FL27 obtained from Phaseolus vulgaris L. as Rhizobium gallicum.

It is proposed that the Austrian isolates and the Mexican strain FL27 are members of the species R. gallicum, and ribosomal gene organization was studied by Southern hybridization with the 16S rRNA gene and temperature gradient gel electrophoresis, indicating identical organizations and the presence of three identical 16S RRNA copies in the genome of this species.

Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees.

A new Rhizobium species that nodulates Phaseolus vulgaris L. and Leucaena spp. is proposed on the basis of the results of multilocus enzyme electrophoresis, DNA-DNA hybridization, an analysis of

Signaling and host range variation in nodulation.

The common and species-specific structural nod genes are involved in the synthesis of specific lipo-oligosaccharides that signal back to the plant to elicit root-hair deformations, cortical-cell divisions, and nodule-meristem formation.

Host plant effect on competition among strains of Rhizobium leguminosarum

Compitiveness for pea nodulation was correlated with an ability to catabolize homoserine, an amino acid found in large quantities in pea root exudate, which indicated a strong preference of both peas and faba beans for strains having certain specific plasmid profiles.

Structural and functional conservation of the rhizopine catabolism (moc) locus is limited to selected Rhizobium meliloti strains and unrelated to their geographical origin.

It is suggested that the use of moc genes as a selectable marker trait for tracking genetically manipulated organisms is feasible.