Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions.

  title={Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions.},
  author={K. Dale Noel and Alma Christina S{\'a}nchez and Leonor Guadarrama Fern{\'a}ndez and J. Leemans and Miguel A Cevallos},
  journal={Journal of bacteriology},
  volume={158 1},
Rhizobium phaseoli CFN42 DNA was mutated by random insertion of Tn5 from suicide plasmid pJB4JI to obtain independently arising strains that were defective in symbiosis with Phaseolus vulgaris but grew normally outside the plant. When these mutants were incubated with the plant, one did not initiate visible nodule tissue (Nod-), seven led to slow nodule development (Ndv), and two led to superficially normal early nodule development but lacked symbiotic nitrogenase activity (Sna-). The Nod… CONTINUE READING
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