Rhizobium leguminosarum cytochromes (Vicia faba)

Abstract

1. By difference spectrophotometry the cytochrome pattern of two strains of Rhizobium leguminosarum — effective and partly-effective — was studied in pure culture and in bacteroids isolated from legume root nodules The term bacteroids is applied to all forms of the specific strain of bacteria isolated from root nodules. . 2. In pure culture both strains contained cytochromes c, b and a-a 3 as well as cytochrome a 2 which appeared under semi-anaerobic conditions, especially in the effective strain. In Rhizobia cytochrome a 2 was detected for the first time. 3. Bacteroids of the effective strain contained cytochromes c, b and some CO-reactive pigments which remained incompletely identified. Bacteroids of the partly-effective strain contained cytochrome a (a = peak at 597 nm at − 196°) which was absent from bacteroids of the effective strain. 4. The data obtained show that the low O2 concentration within nodules does not seem to be the only factor responsible for the absence of cytochromes a from N2-fixing bacteroids. By difference spectrophotometry the cytochrome pattern of two strains of Rhizobium leguminosarum — effective and partly-effective — was studied in pure culture and in bacteroids isolated from legume root nodules The term bacteroids is applied to all forms of the specific strain of bacteria isolated from root nodules. . The term bacteroids is applied to all forms of the specific strain of bacteria isolated from root nodules. In pure culture both strains contained cytochromes c, b and a-a 3 as well as cytochrome a 2 which appeared under semi-anaerobic conditions, especially in the effective strain. In Rhizobia cytochrome a 2 was detected for the first time. Bacteroids of the effective strain contained cytochromes c, b and some CO-reactive pigments which remained incompletely identified. Bacteroids of the partly-effective strain contained cytochrome a (a = peak at 597 nm at − 196°) which was absent from bacteroids of the effective strain. The data obtained show that the low O2 concentration within nodules does not seem to be the only factor responsible for the absence of cytochromes a from N2-fixing bacteroids.

DOI: 10.1007/BF00264179

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@article{Kretovich2004RhizobiumLC, title={Rhizobium leguminosarum cytochromes (Vicia faba)}, author={W. L. Kretovich and Vasilly I. Romanov and Aleksander V. Korolyov}, journal={Plant and Soil}, year={2004}, volume={39}, pages={619-634} }