Revisiting the Identification and cDNA Cloning of T Cell-Replacing Factor/Interleukin-5

  title={Revisiting the Identification and cDNA Cloning of T Cell-Replacing Factor/Interleukin-5},
  author={Kiyoshi Takatsu},
  journal={Frontiers in Immunology},
  • K. Takatsu
  • Published 23 November 2014
  • Biology
  • Frontiers in Immunology
This is a perspective based on the paper “Cloning of complementary DNA encoding T cell-replacing factor and identity with B cell growth factor II,” by Kinashi et al. (1). We have been interested in understanding the molecular basis of T–B cell cooperation for antibody formation. Although many investigators had described a number of different soluble factors that appeared to have biological relevance to T–B cell interactions, molecular basis of such active substances remained unknown for a long… 
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Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II
To elucidate the molecular properties of TRF, cDNA encoding TRF was isolated from the 2.19 T-cell line and report here the structure and multiple activities of this lymphokine.
Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin
The molecular cloning, structural analysis and functional expression of the cDNA encoding human B SF-2 indicated that BSF-2 is functionally and structurally unlike other known proteins.
Identification of a T cell-derived b cell growth factor distinct from interleukin 2
A factor found in induced supernatants of the mouse thymoma EL4 that co-stimulates with anti-IgM antibodies in short-term cultures of purified B lymphocytes to induce polyclonal B cell proliferation but not antibody-forming cell production is reported.
Production of a monoclonal antibody useful in the molecular characterization of murine T-cell-replacing factor/B-cell growth factor II.
The results indicate that monoclonal antibody TB13 recognizes a molecule that has both TRF and BCGF-II activities, which appears to be associated with the same molecule.
Replacement of T-cell function by a T-cell product.
The existence of two distinct factors, one of which can completely replace T-cells, is reported, which is believed to be based on the heavy antigenic stimulation provided by the histocompatibility antigens carried by the B-cells.
Interleukin 4 (B-cell growth factor II/eosinophil differentiation factor) is a mitogen and differentiation factor for preactivated murine B lymphocytes.
It is found that purified interleukin 4 has no effects on small resting B cells but induces naturally occurring large B cells to synthesize DNA and to secrete IgM and low levels of IgG.
Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5.
It is established that a single cDNA clone encodes a protein that acts as a growth and differentiation factor for both B cells and eosinophils.
T Cell‐Replacing Factor (TRF)/Interleukin 5 (IL‐5): Molecular and Functional Properties
The translation product of murine TRF/IL-5 cDNA triggers resting as well as activated murine B cells for terminal differentiation into Ig-secreting cells (IgM, IgG1, or IgA) accompanied by increased mRNA expression for secreted forms of relevant Ig heavy chain (mu, gamma, or alpha).
Establishment of three PPD-reactive helper T cell clones with distinct functions in B cell activation.
It is suggested that B cells can be triggered by at least two distinct helper T cell subpopulations via respective pathways (cognate interaction and factor-mediated interaction) and at least three distinctly functioning PPD-reactive helper T Cells can be generated by active immunization with Tbc in vivo.