Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H+ Ions and Warrants Further Studies of Organellar pH

@inproceedings{urawik2016RevisitingMP,
  title={Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H+ Ions and Warrants Further Studies of Organellar pH},
  author={Tomasz Michał Żurawik and Adam Pomorski and Agnieszka Belczyk-Ciesielska and Grażyna Goch and Katarzyna Niedźwiedzka and Roza Kucharczyk and Artur Krężel and Wojciech Bal},
  booktitle={PloS one},
  year={2016}
}
Fluorescence measurements of pH and other analytes in the cell rely on accurate calibrations, but these have routinely used algorithms that inadequately describe the properties of indicators. Here, we have established a more accurate method for calibrating and analyzing data obtained using the ratiometric probe 5(6)-carboxy-SNARF-1. We tested the… CONTINUE READING