Revisit the Correlation between the Elastic Mechanics and Fusion of Lipid Membranes

@article{Fan2016RevisitTC,
  title={Revisit the Correlation between the Elastic Mechanics and Fusion of Lipid Membranes},
  author={Zih-An Fan and Kuan-yu Tsang and Si-Han Chen and Yi-Fan Chen},
  journal={Scientific Reports},
  year={2016},
  volume={6}
}
Membrane fusion is a vital process in key cellular events. The fusion capability of a membrane depends on its elastic properties and varies with its lipid composition. It is believed that as the composition varies, the consequent change in C0 (monolayer spontaneous curvature) is the major factor dictating fusion, owing to the associated variation in GEs (elastic energies) of the fusion intermediates (e.g. stalk). By exploring the correlations among fusion, C0 and Kcp (monolayer bending modulus… 
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References

SHOWING 1-10 OF 44 REFERENCES
The Gaussian curvature elastic energy of intermediates in membrane fusion.
TLDR
The results show that models of fusion intermediate energy are accurate enough to make semiquantitative predictions about how proteins mediate biomembrane fusion.
Field theoretic study of bilayer membrane fusion. I. Hemifusion mechanism.
TLDR
The results on the architecture and tension needed for successful fusion are summarized in a phase diagram and the barrier to formation of the initial stalk is much smaller than previously estimated by phenomenological theories.
Specific lipids supply critical negative spontaneous curvature--an essential component of native Ca2+-triggered membrane fusion.
TLDR
The Ca(2+)-triggered merger of two apposed membranes is the defining step of regulated exocytosis, and different fusion sites or vesicle types can use specific alternate lipidic components, or combinations thereof, to facilitate and modulate the fusion pore.
The gaussian curvature elastic modulus of N-monomethylated dioleoylphosphatidylethanolamine: relevance to membrane fusion and lipid phase behavior.
TLDR
This is the first determination of the saddle splay (Gaussian) modulus in a lipid system consisting only of phospholipids, and rationalizes the action of fusion-catalyzing proteins in stabilizing nascent fusion pores in biomembranes.
Mechanics of membrane fusion
TLDR
The conserved fusion-through-hemifusion pathway of merger between biological membranes is discussed and it is proposed that the entire progression, from the close juxtaposition of membrane bilayers to the expansion of a fusion pore, is controlled by protein-generated membrane stresses.
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