Revised method for routine determination of urinary dialkyl phosphates using gas chromatography-mass spectrometry.

@article{Ueyama2010RevisedMF,
  title={Revised method for routine determination of urinary dialkyl phosphates using gas chromatography-mass spectrometry.},
  author={Jun Ueyama and Michihiro Kamijima and Takaaki Kondo and K. Takagi and Eiji Shibata and Takaaki Hasegawa and Shinya Wakusawa and Tomoko Taki and Masahiro Gotoh and Isao Saito},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2010},
  volume={878 17-18},
  pages={1257-63}
}
Among urinary organophosphorus pesticide (OP) metabolites, dialkyl phosphates (DAPs) have been most often measured as a sensitive biomarker in non-occupational and occupational OP exposure risk assessment. In our conventional method, we have employed a procedure including simple liquid-liquid extraction (diethyl ether/acetonitrile), derivatization (pentafluorobenzylbromide, PFBBr) and clean-up (multi-layer column) for gas chromatography-mass spectrometry (GC-MS) analysis starting from 5-mL… CONTINUE READING