Revised Taxonomy of the Methanotrophs: Description of Methylobacter gen. nov., Emendation of Methylococcus, Validation of Methylosinus and Methylocystis Species, and a Proposal that the Family Methylococcaceae Includes Only the Group I Methanotrophs

  title={Revised Taxonomy of the Methanotrophs: Description of Methylobacter gen. nov., Emendation of Methylococcus, Validation of Methylosinus and Methylocystis Species, and a Proposal that the Family Methylococcaceae Includes Only the Group I Methanotrophs},
  author={John P. Bowman and Lindsay I. Sly and Peter D. Nichols and Alice Hayward},
  journal={International Journal of Systematic and Evolutionary Microbiology},
  • J. Bowman, L. Sly, A. Hayward
  • Published 1 October 1993
  • Biology
  • International Journal of Systematic and Evolutionary Microbiology
Numerical taxonomic, DNA-DNA hybridization, and phospholipid fatty acid composition analyses were performed on an extensive range of methanotrophic strains, including reference strains and environmental isolates obtained from sites throughout eastern Australia. When the results of these studies were related to the results of a study based on genomic physicochemical properties, they clarified group I and II methanotroph genus and species interrelationships. The group I methanotrophs were found… 

Figures and Tables from this paper

Classification of halo(alkali)philic and halo(alkali)tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus Methylomicrobium.
Based on the results presented here and on previously reported morphological and physiological characteristics, these halotolerant and halophilic methanotrophic strains are classified as representing novel species within the genus Methylomicrobium.
Phylogenomic Analysis of the Gammaproteobacterial Methanotrophs (Order Methylococcales) Calls for the Reclassification of Members at the Genus and Species Levels
The order Methylococcales constitutes the methanotrophs – bacteria that can metabolize methane, a potent greenhouse gas, as their sole source of energy. These bacteria are significant players in the
Family- and Genus-Level 16S rRNA-Targeted Oligonucleotide Probes for Ecological Studies of Methanotrophic Bacteria
The broad coverage and high degree of specificity of this new suite of probes will provide more detailed, quantitative information about the community structure of methanotrophs in environmental samples than was previously available.
Analysis of 16S rRNA and methane monooxygenase gene sequences reveals a novel group of thermotolerant and thermophilic methanotrophs, Methylocaldum gen. nov.
It is proposed that strains OR2 and LK6, together with the misclassified thermophilic strains Methylomonas gracilis VKM-14LT and Methylococcus thermophilus IMV-B3122, comprise a new genus of thermophile methanotrophs, Methylocaldum gen. nov., containing three new species.
Pan-Genome-Based Analysis as a Framework for Demarcating Two Closely Related Methanotroph Genera Methylocystis and Methylosinus
The phylogenomic analysis confirmed that members of the Methylocystis/Methylosinus clade form two separate clades, and major differences between the genome-encoded environmental traits of these methanotrophs include a variety of enzymes for methane oxidation and dinitrogen fixation as well as genomic determinants for cell motility and photosynthesis.
Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer.
The isolate of CSC1(T), a Gram-negative, aerobic, methane-oxidizing bacterium, was isolated from an uncontaminated aquifer nearly 20 years ago is proposed as a member of a novel species of the genus Methylocystis, Methylcystis hirsuta sp.
A novel methanotroph in the genus Methylomonas that contains a distinct clade of soluble methane monooxygenase
A novel methanotroph designated EMGL16-1 that was isolated from a freshwater lake using the floating filter culture technique was found to be closely related to the genus Methylomonas in the family Methylococcaceae of the class Gammaproteobacteria.
Soluble Methane Monooxygenase Gene Clusters from Trichloroethylene-Degrading Methylomonas sp. Strains and Detection of Methanotrophs during In Situ Bioremediation
A new PCR primer was successfully used for detecting methanotrophs in the groundwater of trichloroethylene-contaminated sites during in situ-biostimulation treatments and the phylogenetic analysis of the predicted amino acid sequences of sMMO demonstrated that the sM MOs from these strains were closer to that from M. capsulatus Bath in the group X methanOTrophs.
Characterization of the first rice paddy cluster I isolate, Methyloterricola oryzae gen. nov., sp. nov. and amended description of Methylomagnum ishizawai.
Three gammaproteobacterial methanotrophic strains (73aT, 175 and 114) were isolated from stems of rice plants and showed that strains 114 and 175 are most closely related to Methylomagnum ishizawai, a new genus within the family Methylococcaceae.


Contribution of Genome Characteristics to Assessment of Taxonomy of Obligate Methanotrophs
The DNAs of a variety of obligate methanotrophic bacteria were analyzed for base composition and nucleotide distribution and genome molecular weight showed that related species formed distinct clusters.
Taxonomic Studies on Some Gram-negative Methylotrophic Bacteria
The results indicate that the obligate methanol-utilizing bacteria all exhibit a high phenotypic similarity and are taxonomically distinct from the restricted facultative and the facultative methnol- utilizing bacteria examined and from the pseudomonad reference strains.
16S ribosomal RNA sequence analysis for determination of phylogenetic relationship among methylotrophs.
The results demonstrate that it is possible to distinguish and classify the methylotrophic bacteria using 16S rRNA sequence analysis.
[Isolation and properties of new strains of obligate methanotrophs].
New strains of obligate methanotrophic bacteria which assimilate only methane or methanol as the source of carbon and energy have been isolated. According to their morphology, ultrastructure,
Characterization of the methanotrophic bacterial community present in a trichloroethylene-contaminated subsurface groundwater site
The hypothesis that stimulation of indigenous methanotrophic communities can be efficacious for removal of chlorinated aliphatic hydrocarbons from subsurface sites and that the removal can be mediated by sMMO is partially supported.
Membrane fatty acids as phenotypic markers in the polyphasic taxonomy of methylotrophs within the Proteobacteria.
The PLFA phenotype relationships compare favourably with phylogenetic associations based on 16S rRNA data for methylotrophs and will continue to be a valuable phenotypic marker for Proteobacteria taxonomy.
Numerical analysis of the protein electrophoregrams of obligate methanotrophic bacteria
The protein spectra for 70 strains of obligate methanotrophic bacteria were studied using electrophoresis in polyacrylamide gel with sodium dodecyl sulphate, and the data suggest the existence of 5 independent genera of obligates: Methylomonas, Methylobacter, M methylococcus, Mmethylosinus and Methylocystis.
Taxonomic studies on methylotrophic bacteria by 5S ribosomal RNA sequencing.
Nucleotide sequences of 5S ribosomal RNA isolated from 19 strains of Gram-negative methylotrophic bacteria showed that the bacteria analysed belong to the Proteobacteria and fell into several clusters, including obligate methanotrophics, obligate methylotrophs and several groups of facultative methylotophs.
Contribution to the taxonomy of methanotrophic bacteria: Correlation between membrane type and GC‐value
Thirty‐nine isolates and additional reference strains of methanotrophic bacteria were studied with respect to mol % G + C of their DNA and the type of intracytoplasmatic membranes. The results on the