Revised Guidance Document 150 on Standardised Test Guidelines for Evaluating Chemicals for Endocrine Disruption

  title={Revised Guidance Document 150 on Standardised Test Guidelines for Evaluating Chemicals for Endocrine Disruption},
  author={R eised},
  journal={OECD Series on Testing and Assessment},
  • R. eised
  • Published 3 September 2018
  • OECD Series on Testing and Assessment
Regulatory Testing for Endocrine Disruptors; Need for Validated Methods and Integrated Approaches
The current testing of substances regarding their potential endocrine disrupting properties is hampered by a lack of validated methods. Indeed only a few endocrine pathways can be investigated by
Reproduction/Developmental Toxicity Screening Test (OECD TG 421) and Combined Repeated Dose Toxicity Study with the Reproduction/Developmental Toxicity Screening Test (OECD TG 422)
  • Tg
  • OECD Series on Testing and Assessment
  • 2018
Weights of: (parents only) testes, epididymides, prostate and seminal vesicles with coagulating glands (OECD TG 421 and TG 422), plus adrenals (OECD TG 422 only). Optional organ weights could include
A Novel, Benchmark-Centered, Eco-Impact Rating System for Sunscreens and Sunscreen Formulation Design
Ultraviolet (UV) filter compounds are the key constituents in sunscreens that protect human skin from detrimental solar radiation. Some of these products have attracted public attention due to claims
Developmental exposure to the DE-71 mixture of polybrominated diphenyl ether (PBDE) flame retardants induce a complex pattern of endocrine disrupting effects in rats
Polybrominated diphenyl ethers (PBDEs) are legacy compounds with continued widespread human exposure. Despite this, developmental toxicity studies of DE-71, a mixture of PBDEs, are scarce and its
An overview of current practices for regulatory risk assessment with lessons learnt from cosmetics in the European Union
The present review aims to provide an overview of established and more recently introduced methods for hazard assessment and risk characterisation for human health in the context of the EU Cosmetics Regulation as well as the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) Regulation.
Application of an in Vitro Assay to Identify Chemicals That Increase Estradiol and Progesterone Synthesis and Are Potential Breast Cancer Risk Factors
In vitro screening data is a helpful addition to current toxicity assessments, which are not sensitive to mammary gland effects, and several hundred chemicals that should be considered as potential risk factors for breast cancer because they increased E2 or P4 synthesis are identified.
Assessment of the endocrine disrupting properties of bisphenol AF: a case study applying the European regulatory criteria and guidance
A case study was performed on Bisphenol AF to explore the application of the EU criteria and EFSA/ECHA guidance document for the ED assessment of a non-pesticide chemical regulated under REACH and indicated that BPAF meets the ED criteria for human health.
Classical toxicity endpoints in female rats are insensitive to the human endocrine disruptors diethylstilbestrol and ketoconazole.
There is a need for more sensitive test methods to protect the reproductive health of girls and women from harmful chemicals, and endocrine disrupting effects on sensitive endpoints in male offspring are observed.
Comparison of In Vitro Endocrine Activity of Phthalates and Alternative Plasticizers
Compared the in vitro endocrine activity of several already used potential alternative plasticizers or new substitutes to one of 2 phthalates, DEHP and DINP, and effects of these chemicals on 3 common mechanisms of endocrine disruption were studied.
Curated Data In — Trustworthy In Silico Models Out: The Impact of Data Quality on the Reliability of Artificial Intelligence Models as Alternatives to Animal Testing
It is asserted that data curation is a critical step in building computational models, to help ensure that reliable predictions of chemical toxicity are achieved through use of the models.


Development of a QSAR Model for Thyroperoxidase Inhibition and Screening of 72,526 REACH substances
REACH substances DTU Orbit (07/12/2018) Development of a QSAR Model for Thyroperoxidase Inhibition and Screening of 72,526 REACH substances Thyroid hormones (THs) are involved in multiple biological
Environmentally induced epigenetic toxicity: potential public health concerns
The present understanding of epigenetic mechanisms involved in the mammalian life cycle is examined, the current evidence for environmentally induced epigenetic toxicity in human cohorts and rodent models is evaluated, and the research considerations and implications of this emerging knowledge for public health and regulatory toxicology are highlighted.
Scientific principles for the identification of endocrine-disrupting chemicals: a consensus statement
The difficulty in retrospectively reconstructing ED exposure, insufficient range of validated test systems for EDs, and some issues impacting on the evaluation of the risk from EDs are highlighted, such as non-monotonic dose–response and thresholds, modes of action, and exposure assessment.
Environmentally Induced Epigenetic Transgenerational Inheritance of Reproductive Disease1
This review describes the studies in a variety of species that impact reproductive disease and abnormalities and suggests serious attention be paid to the possibility that ancestral exposures to environmental insults promotes transgenerational inheritance of reproductive disease susceptibility.
Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish.
Investigation of whether chronic exposure to contaminants experienced by wild female fish throughout their juvenile phase can affect the DNA methylation status of their oocytes during gonad maturation found a higher gonad growth was observed in fish from the reference site in comparison to contaminated fish.
Development of a thyroperoxidase inhibition assay for high-throughput screening.
The development of a HTPS TPO inhibition assay that was used to screen large numbers of chemicals as an integral component of a tiered TH-disruptor screening approach and demonstrated the capacity of this assay to detect diverse TPO inhibitors.
The two faces of janus: sex steroids as mediators of both cell proliferation and cell death.
It is reported that prolonged estrogen withdrawal in breast cancer cells results in the acquisition of an apoptotic response to 17 -estradiol; i.e., the very same hormone that mediates the proliferation of its target cells in the female genital tract and mammary glands is also able to trigger their death.
Several environmental oestrogens are also anti-androgens.
It is shown that many of the so-called 'environmental oestrogens' also possess anti-androgenic activity, demonstrating that hormone-mimicking chemicals can have multiple hormonal activities, which may make it difficult to interpret their mechanisms of action in vivo.
Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen
An estrogen-inducible screen was developed in yeast (Saccharomyces cerevisiae) in order to assess whether surfactants and their major degradation products are estrogenic. The DNA sequence of the
“ Guidance document on the validation and international acceptance of new or updated test methods for hazard assessment ”