Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

@article{Jones2005ReviewOM,
  title={Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat},
  author={Huw D Jones and Angela Doherty and Huixia Wu},
  journal={Plant Methods},
  year={2005},
  volume={1},
  pages={5 - 5}
}
Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties. 

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RIS: Agrobacterium tumefaciens-mediated transformation of wheat using suspension cells as a model system and green fluorescent protein as a visual marker

  • B Weir, X Gu, MB Wang, N Upadhyaya, AR Elliott, Brettell
  • Aust J Plant Physiol
  • 2001
Highly Influential
6 Excerpts

Wheat transformation: current technology and applications to grain development and composition

  • HD Jones
  • J Cereal Sci
  • 2005
2 Excerpts

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