Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest in Agave L. for genetic improvement and biocatalysis

  title={Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest in Agave L. for genetic improvement and biocatalysis},
  author={M. Tamayo-Ord{\'o}{\~n}ez and B. Ayil-Guti{\'e}rrez and Y. Tamayo-Ord{\'o}{\~n}ez and L. Rodr{\'i}guez-Zapata and M. Monforte-Gonz{\'a}lez and E. A. De la Cruz-Arguijo and M. J. Garc{\'i}a-Castillo and L. S{\'a}nchez-Teyer},
  journal={Biotechnology Progress},
Several of the over 200 known species of Agave L. are currently used for production of distilled beverages and biopolymers. The plants live in a wide range of stressful environments as a result of their resistance to abiotic stress (drought, salinity, and extreme temperature) and pathogens, which gives the genus potential for germplasm conservation and biotechnological applications that may minimize economic losses as a result of the global climate change. However, the limited knowledge in the… Expand
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Interspecific evolutionary relationships of alpha-glucuronidase in the genus Aspergillus.
3D structure modeling showed both conservation and differences in amino acid residues participating in enzyme-substrate binding, which indicates the possibility that some enzymes are functionally specialized for the specific degradation of a substrate depending on the genetics of each species in the genus and the condition of the habitat where they evolved. Expand
Agave: A natural renewable resource with multiple applications.
The economic base of Agave plants can be expanded if expended biomass could be transformed into products that are useful for applications in food, forage, ensilage, agriculture, medicine; energy, environment, textiles, cosmetics, and aesthetics. Expand
Exohidrolasas fructosílicas y su importancia en el metabolismo de fructanos en Agave tequilana Weber var. azul
Fructans are carbohydrates of diverse chemical structure that are distributed in different taxa. In plants, in addition to be an important source of carbon, they have been associated with toleranceExpand
Comparison of dilute acid pretreatment of agave assisted by microwave versus ultrasound to enhance enzymatic hydrolysis.
A comparison between microwave and ultrasound irradiations in the agave pretreatment using dilute sulfuric acid as catalyst was assessed and showed that under optimal conditions, the hemicellulose removal was superior in the pretreatment assisted with microwave compared to ultrasound. Expand


Advances in culture and genetic modification approaches to lipid biosynthesis for biofuel production and in silico analysis of enzymatic dominions in proteins related to lipid biosynthesis in algae
Based on primary amino acid sequences and tertiary structure of proteins shared by certain algae, it is likely that these proteins may relate to lipid yield and accumulation, which makes bioinformatics a powerful tool for in silico study of proteins and for selecting genes involved in lipid biosynthesis that could be useful for heterologous transformation in algae. Expand
Development of Agave as a dedicated biomass source: production of biofuels from whole plants
Agave’s potential to be a source of fermentable sugars beyond the existing beverage species to now include many species previously unfermentable by yeast, including cold-tolerant lines, should stimulate development of Agave as a dedicated feedstock for biofuels in semi-arid regions throughout the globe. Expand
De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
This work presents a comprehensive transcriptome resource for two Agave species and provides insight into their biology and physiology, and comparison of agave proteomes to those of additional plant species identifies biological functions of gene families displaying sequence divergence in agave species. Expand
Increased Production of Nutriments by Genetically Engineered Crops
Examples of plant genetic modifications with possible positive effects on human nutrition are described, showing how the metabolism of primary constituents of the plant cell such as sugar or amino acids, but also of secondary metabolites such as flavonoids, can be modified by genetic engineering. Expand
Bioconversion of Agave tequilana fructans by exo-inulinases from indigenous Aspergillus niger CH-A-2010 enhances ethanol production from raw Agave tequilana juice
Observations indicate that fructans from raw Agave tequilana juice can be efficiently hydrolyzed by using A. niger CH-A-2010 inulinases, and that this procedure impacts positively on the production of ethanol. Expand
Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots.
These results demonstrate in planta that the enzymes sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1- fructosytransferase are sufficient to synthesize inulin molecules of all chain lengths naturally occurring in a given plant species. Expand
Potential of Plants from the Genus Agave as Bioenergy Crops
Agave has great potential to be developed as a bioenergy crop due to its capacity to grow with very little rainfall and/or inputs and still reach good amount of biomass, so unused semi-arid land could be productive. Expand
Effects of drought, transgenic expression of a fructan synthesizing enzyme and of mycorrhizal symbiosis on growth and soluble carbohydrate pools in tobacco plants
The effects of three conditions likely to affect soluble carbohydrate pools, namely drought, expression of barley sucrose: fructan 6-fructosyl transferase (6-SFT, EC and the establishmentExpand
Genomic resources and transcriptome mining in Agave tequilana
The current genomic resources available for agave and the potential for transcriptome mining in relation to bioenergy applications are discussed. Expand
Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose:fructan 6‐fructosyltransferase
Functional expression of 6‐SFT from barley in transgenic tobacco and chicory is reported, particularly the tetrasaccharide bifurcose which is also a main fructan in barley leaves. Expand