Review—Progress in concept and practice of growing anchorage-dependent mammalian cells in three dimension

@article{Rappaport2003ReviewProgressIC,
  title={Review—Progress in concept and practice of growing anchorage-dependent mammalian cells in three dimension},
  author={Catherine Rappaport},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={2003},
  volume={39},
  pages={187-192}
}
Tissue culture has played a major role in the rapid advances made in medical science in the past 50 yr. The full potential of the technique, however, is limited by the fact that growth of cells is usually restricted to a monolayer accompanied by major decreases in many of their tissue-specific functions. This has been shown to be due, in large part, to the inadequate oxygenation of cells growing in tissue culture dishes. Studies that show that the high charge density and rigidity of the plastic… CONTINUE READING