Reversion analysis of dynein intermediate chain function.

@article{Mitchell1993ReversionAO,
  title={Reversion analysis of dynein intermediate chain function.},
  author={David R. Mitchell and Y. Kang},
  journal={Journal of cell science},
  year={1993},
  volume={105 ( Pt 4)},
  pages={1069-78}
}
The ODA6 locus of Chlamydomonas reinhardtii encodes a 70 kDa intermediate chain protein of the flagellar outer row dynein ATPase, and mutations at this locus prevent assembly of the entire outer row dynein arm complex. To initiate a structure-function analysis of the 70 kDa protein, we used transformation with chimeric mutant/wild-type genes to localize the defect in one assembly mutation, oda6-95. Sequence analysis revealed a frame-shift mutation in codon 53, which is followed by a stop codon… CONTINUE READING

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