Reversible vacuolation of the transverse tubules of frog skeletal muscle: a confocal fluorescence microscopy study

@article{Krolenko1995ReversibleVO,
  title={Reversible vacuolation of the transverse tubules of frog skeletal muscle: a confocal fluorescence microscopy study},
  author={Sergei A. Krolenko and W. Brad Amos and Jack A. Lucy},
  journal={Journal of Muscle Research & Cell Motility},
  year={1995},
  volume={16},
  pages={401-411}
}
A confocal microscope was used to investigate the reversible vacuolation of frog skeletal muscle fibres produced by the efflux and entry of glycerol (80–100 mm). The formation, development and disappearance of vacuoles was observed in the fibres by staining simultaneously with two fluorescent membrane probes, RH414 and DiOC6(3). The styryl dye, RH414, stains only the plasmalemma and the membranes of the transverse tubules. In normal and glycerol-loaded fibres, RH414 revealed regular, narrow… CONTINUE READING

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