Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the hemoglobins of the domestic cat: acetylation of NH3+ terminal group of the beta chain transforms the complex pH dependence of the forward apparent second order rate constant to a simple form.

@article{Okonjo2006ReversibleRO,
  title={Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the hemoglobins of the domestic cat: acetylation of NH3+ terminal group of the beta chain transforms the complex pH dependence of the forward apparent second order rate constant to a simple form.},
  author={Kehinde Onwochei Okonjo and Adedayo A Fodeke},
  journal={Biophysical chemistry},
  year={2006},
  volume={119 2},
  pages={196-204}
}
We demonstrate kinetically that the reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9[93]beta sulfhydryl group of domestic cat hemoglobins is a reversible process. In the major hemoglobin, in which the NH3+ terminal group of GlyNA1[1]beta is free, kf, the apparent forward second order rate constant, has a complex pH dependence profile. In the minor hemoglobin, the NH3+ terminal group of SerNA1[1]beta is acetylated, and the pH dependence profile of kf is simple. These results support… CONTINUE READING
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Babarinde, Effect of A3[6]h mutation on the reactivity of CysF9[93]h sulfhydryl group of human haemoglobin

  • K. O. Okonjo, Y. A. Aken’ova, C. O. Aboluwoye, S. Nwozo, F. U. Akhigbe, N.A.J.O. Babalola
  • S, J. Chem. Soc., Faraday Trans
  • 1996
Highly Influential
10 Excerpts

Organic phosphate-binding groups electrostatically linked to the reactivity of the CysF9[93]h sulfhydryl group of haemoglobin

  • K. O. Okonjo, C. O. Aboluwoye, J. O. Babalola, I. A. Usanga
  • J. Chem. Soc., Faraday Trans. 91
  • 1995
Highly Influential
10 Excerpts

Structure-reactivity correlations for the thiol-disulfide interchange reaction

  • J. M. Wilson, R. J. Bayer, D. J. Hupe
  • J. Am. Chem. Soc. 99 (1977) 7922–7926. K.O…
  • 2006
1 Excerpt

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