Reversible mitotic and metabolic inhibition following the encapsulation of fibroblasts in alginate hydrogels.

@article{Hunt2009ReversibleMA,
  title={Reversible mitotic and metabolic inhibition following the encapsulation of fibroblasts in alginate hydrogels.},
  author={Nicola Claire Hunt and Richard M. Shelton and Liam M Grover},
  journal={Biomaterials},
  year={2009},
  volume={30 32},
  pages={6435-43}
}
Limiting cell proliferation without reducing cell viability for in vivo tissue engineering applications is important in co-culture applications where the growth of one cell type must be inhibited to prevent overgrowth of the scaffold at the expense of another cell type. Also, it is vital for maintaining viability of cells in large constructs before vascularisation occurs. In this study we have shown by means of the Thiazolyl blue (MTT) assay and immuno-staining for proliferating cell nuclear… CONTINUE READING

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