Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants.

@article{Matzke1989ReversibleMA,
  title={Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants.},
  author={Marjori Matzke and M Primig and J Trnovsk{\'y} and Antonius J. M. Matzke},
  journal={The EMBO journal},
  year={1989},
  volume={8 3},
  pages={643-9}
}
Doubly transformed tobacco plants were obtained following sequential transformation steps using two T-DNAs encoding different selection and screening markers: T-DNA-I encoded kanamycin resistance and nopaline synthase; T-DNA-II encoded hygromycin resistance and octopine synthase. A genetic analysis of the inheritance of the selection and screening marker genes in progeny of the doubly tranformed plants revealed that the expression of T-DNA-I genes was often suppressed. This suppression could be… CONTINUE READING
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