Reversible immobilization of engineered molecules by Ni-NTA chelators.


Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array.

Cite this paper

@article{Maly2004ReversibleIO, title={Reversible immobilization of engineered molecules by Ni-NTA chelators.}, author={J Maly and C Di Meo and M De Francesco and A Masci and J Masojidek and M Sugiura and A Volpe and R Pilloton}, journal={Bioelectrochemistry}, year={2004}, volume={63 1-2}, pages={271-5} }