Reversible hypercondensation and decondensation of mitotic chromosomes studied using combined chemical-micromechanical techniques.

@article{Poirier2002ReversibleHA,
  title={Reversible hypercondensation and decondensation of mitotic chromosomes studied using combined chemical-micromechanical techniques.},
  author={Michael Guy Poirier and Tamar Monhait and John Frederick Daniel Marko},
  journal={Journal of cellular biochemistry},
  year={2002},
  volume={85 2},
  pages={422-34}
}
We show that the chromatin in mitotic chromosomes can be drastically overcompacted or unfolded by temporary shifts in ion concentrations. By locally 'microspraying' reactants from micron-size pipettes, while simultaneously monitoring the size of and tension in single chromosomes, we are able to quantitatively study the dynamics of these reactions. The tension in a chromosome is monitored through observation and calibration of bending of the glass pipettes used to manipulate the chromosomes. For… CONTINUE READING
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