Reversible dissociation of gamma-glutamylcysteine synthetase into two subunits.

  title={Reversible dissociation of gamma-glutamylcysteine synthetase into two subunits.},
  author={G F Seelig and Royce P. Simondsen and Alton Meister},
  journal={The Journal of biological chemistry},
  volume={259 15},
gamma-Glutamylcysteine synthetase (rat kidney; Mr approximately 104,000) is composed of 2 nonidentical subunits. In the present work, a procedure was developed for the reversible dissociation of the enzyme into its subunits (Mr = 73,000 and 27,700) under nondenaturing conditions. Students in which gel electrophoresis was used, in conjunction with an enzyme activity stain and elution and re-electrophoresis of protein bands, showed that the heavy subunit contains all of the structural… CONTINUE READING
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