Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators.

@article{Kotera2010ReversibleDO,
  title={Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators.},
  author={Ippei Kotera and Takuya Iwasaki and Hiromi Imamura and Hiroyuki Noji and Takeharu Nagai},
  journal={ACS chemical biology},
  year={2010},
  volume={5 2},
  pages={215-22}
}
Fluorescent protein (FP)-based Forster resonance energy transfer (FRET) technology is useful for development of functional indicators to visualize second messenger molecules and activation of signaling components in living cells. However, the design and construction of the functional indicators require careful optimization of their structure at the atomic level. Therefore, routine procedures for constructing FRET-based indicators currently include the adjustment of the linker length between the… CONTINUE READING