Reversible compartmentalization of de novo purine biosynthetic complexes in living cells.

@article{An2008ReversibleCO,
  title={Reversible compartmentalization of de novo purine biosynthetic complexes in living cells.},
  author={Songon An and Ravindra Kumar and Erin D. Sheets and Stephen J Benkovic},
  journal={Science},
  year={2008},
  volume={320 5872},
  pages={103-6}
}
Purines are synthesized de novo in 10 chemical steps that are catalyzed by six enzymes in eukaryotes. Studies in vitro have provided little evidence of anticipated protein-protein interactions that would enable substrate channeling and regulation of the metabolic flux. We applied fluorescence microscopy to HeLa cells and discovered that all six enzymes colocalize to form clusters in the cellular cytoplasm. The association and dissociation of these enzyme clusters can be regulated dynamically… CONTINUE READING

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