Reversible and Irreversible Mechanical Damaging of Large Double-Stranded DNA upon Electrospraying.

@article{Shlyapnikov2016ReversibleAI,
  title={Reversible and Irreversible Mechanical Damaging of Large Double-Stranded DNA upon Electrospraying.},
  author={Yuri M. Shlyapnikov and Elena Andreevna Shlyapnikova and Victor N. Morozov},
  journal={Analytical chemistry},
  year={2016},
  volume={88 14},
  pages={
          7295-301
        }
}
Electrohydrodynamic spraying (or electrospaying, ES) of DNA solutions is an attractive technique for applications in mass spectrometry, in microarray fabrication, and in generation of DNA nanoaerosols. Here we report how ES affects DNA structure and evaluate possible ways to reduce DNA damage upon ES. It is shown that under any ES conditions, linear λ-phage DNA is subjected to intensive rupture producing a mixture of fragments. In addition to such fragmentation, notable reversible changes in… CONTINUE READING
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