Reversible MM-creatine kinase binding to cardiac myofibrils.

@article{Venturaclapier1987ReversibleMK,
  title={Reversible MM-creatine kinase binding to cardiac myofibrils.},
  author={R Ventura-clapier and V. Saks and Guy Vassort and Christoph Lauer and Galina V. Elizarova},
  journal={The American journal of physiology},
  year={1987},
  volume={253 3 Pt 1},
  pages={C444-55}
}
Skinned rat papillary muscles and purified preparations of rat cardiac myofibrils were used to study the nature of the interaction of creatine kinase with cardiac myofibrils. High activity of creatine kinase (2 IU/mg protein in fibers and 0.9 IU/mg in purified myofibrils) was due mostly to reversibly bound enzyme. This activity could be removed and rebound. The process of creatine kinase rebinding was characterized by apparent Km value of 0.14 mg/ml (approximately equal to 2 X 10(6) M… CONTINUE READING
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