Reversible, calcium-dependent membrane association of human leukocyte 5-lipoxygenase.

  title={Reversible, calcium-dependent membrane association of human leukocyte 5-lipoxygenase.},
  author={Carol A. Rouzer and Bengt Samuelsson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={84 21},
Maximal activity of human leukocyte 5-lipoxygenase requires Ca2+, ATP, a microsomal membrane preparation, and two cytosolic stimulatory factors. We report here some effects of Ca2+ on the physical properties of the 5-lipoxygenase. When leukocytes were homogenized in the presence of 2 mM EDTA, 5-lipoxygenase was found to be a soluble enzyme. However, when Ca2+ was added to homogenization buffers at 0-1 mM in excess of EDTA, increasing quantities of the enzyme were recovered in the microsomal… CONTINUE READING

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