Reversibility of the Ca(2+) channel alpha(1)-beta subunit interaction.

  title={Reversibility of the Ca(2+) channel alpha(1)-beta subunit interaction.},
  author={Delphine Bichet and Chantal Lecomte and Jean Marc Sabatier and Ricardo F{\'e}lix and Michel De Waard},
  journal={Biochemical and biophysical research communications},
  volume={277 3},
The auxiliary beta subunit importantly regulates voltage-dependent Ca(2+) channel activity through an interaction with the AID domain, a binding site located in the cytoplasmic I-II linker of the ion-conducting alpha(1) subunit. In the present study, we used two synthetic peptides corresponding to partial sequences of the I-II linker of alpha(1A) (AID(A)-peptides) as tools to disrupt the alpha(1)-beta interaction. In vitro binding experiments confirmed that these peptides exhibit a reasonable… CONTINUE READING