Reversibility of estrogenic sex changes in zebrafish (Danio rerio).

  title={Reversibility of estrogenic sex changes in zebrafish (Danio rerio).},
  author={Mia G. G. Larsen and Katrine Bilberg and Erik Baatrup},
  journal={Environmental toxicology and chemistry},
  volume={28 8},
Development of male zebrafish (Danio rerio) courtship behavior was studied following estrogenic disruption of sexual differentiation. Sixty zebrafish were exposed at 28 degrees C to 5 ng/L (nominal concentration) of 17alpha-ethinylestradiol (EE2) from the egg stage until adulthood at four months of age, resulting in a female-biased sex ratio. Twenty-five EE2-exposed phenotypic female zebrafish were subsequently held in clean water for eight months. During this period, eight phenotypic males… CONTINUE READING