Reversed-phase supports for th resolution of large denatured protein fragments.

@article{Pearson1981ReversedphaseSF,
  title={Reversed-phase supports for th resolution of large denatured protein fragments.},
  author={James D Pearson and Walter C. Mahoney and Mark A. Hermodson and Fred E. Regnier},
  journal={Journal of chromatography},
  year={1981},
  volume={207 3},
  pages={325-32}
}
Large pore (much greater than 300 A), spherical partical silica stationary phases possessing either C18 or C8 hydrocarbon ligands out-performed small pore (60-100A), irregular shaped silicas for the purification of large denatured peptides. Since columns 5 cm in length appeared to be as effective in separating peptides as columns 5 times longer, it is likely that large peptides absorb to the matrix rather than partition between the stationary and mobile phases. 

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