Reversed-phase-reversed-phase liquid chromatography approach with high orthogonality for multidimensional separation of phosphopeptides.

@article{Song2010ReversedphasereversedphaseLC,
  title={Reversed-phase-reversed-phase liquid chromatography approach with high orthogonality for multidimensional separation of phosphopeptides.},
  author={Chunxia Song and Mingliang Ye and Guanghui Han and Xinning Jiang and Fangjun Wang and Zhiyuan Yu and Rui Chen and Hanfa Zou},
  journal={Analytical chemistry},
  year={2010},
  volume={82 1},
  pages={53-6}
}
Protein phosphorylation regulates a series of important biological processes in eukaryotes. However, the phosphorylation sites found up to now are far below than that actually exists in proteins due to the extreme complexity of the proteome sample. Here a new reversed-phase-reversed-phase liquid chromatography (RP-RPLC) approach was developed for multidimensional separation of phosphopeptides. In this approach, a large number of fractions were collected from the first dimensional RPLC… CONTINUE READING

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