Reversed-phase liquid chromatography of radiolabeled peptides using a C18 guard-PAK precolumn system.

Abstract

In order to avoid radioactive contamination of high-performance liquid chromatography columns and injectors, we have investigated the use of a Guard-PAK precolumn system for the chromatography of [125I] labeled peptides. Two gonadotropin-releasing hormone analogs: 1) [D-Ala6-des-Gly10]-GnRH (GnRH-[Ala6]) and 2) [D-Ser(TBu)6-des-Gly10]-GnRH (GnRH-[Ser6]) and… (More)

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