Reversed-phase liquid chromatography/mass spectrometry analysis of reduced monoclonal antibodies in pharmaceutics.

@article{Rehder2006ReversedphaseLC,
  title={Reversed-phase liquid chromatography/mass spectrometry analysis of reduced monoclonal antibodies in pharmaceutics.},
  author={Douglas S. Rehder and Thomas M. Dillon and Gary D. Pipes and Pavel V. Bondarenko},
  journal={Journal of chromatography. A},
  year={2006},
  volume={1102 1-2},
  pages={164-75}
}
A reversed-phase LC/MS method was developed for reduced antibodies that provides efficient separation of light chain and two variants of heavy chain containing N-terminal glutamine and pyroglutamic acid. The best separation was achieved on Zorbax CN and Varian Pursuit DiPhenyl columns eluted with increasing percentage of n-propanol and acetonitrile in 0.1% trifluoroacetic acid. Although glutamine was genetically coded for the N-terminal residue of heavy chain of a monoclonal antibody used in… CONTINUE READING

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