Reversed-phase liquid chromatographic determination of enantiomers of atenolol in rat plasma using derivatization with Marfey's reagent.

@article{Bhushan2009ReversedphaseLC,
  title={Reversed-phase liquid chromatographic determination of enantiomers of atenolol in rat plasma using derivatization with Marfey's reagent.},
  author={R. Bhushan and Shivani Tanwar},
  journal={Biomedical chromatography : BMC},
  year={2009},
  volume={23 7},
  pages={
          787-91
        }
}
  • R. Bhushan, Shivani Tanwar
  • Published 2009
  • Chemistry, Medicine
  • Biomedical chromatography : BMC
  • An HPLC method was established for enantioseparation of (R,S)-atenolol (ATE) and determination of enantiomers in rat plasma. Marfey's reagent (1-fluoro-2,4-dinitrophenyl-5-L-alanine amide, FDNP-L-Ala-NH(2), MR) was used as chiral derivatizing reagent with detection of diastereomers at 340 nm. It was shown that the R-isomer eluted before the S-isomer. The method was validated for linearity, repeatability, limits of detection and limit of quantification (LOQ). Recovery of ATE at LOQ was 92.8% for… CONTINUE READING
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