Reversed-phase ion-pair liquid chromatography analysis and purification of small interfering RNA.

@article{McCarthy2009ReversedphaseIL,
  title={Reversed-phase ion-pair liquid chromatography analysis and purification of small interfering RNA.},
  author={Sean M. McCarthy and M Bernal Gilar and John C. Gebler},
  journal={Analytical biochemistry},
  year={2009},
  volume={390 2},
  pages={181-8}
}
Small interfering RNA (siRNA)-induced gene silencing shows great promise in genomic research and therapeutic applications. siRNA duplexes are typically assembled from complementary synthetic oligonucleotides. High-purity single-stranded species are required for in vivo applications. Methods for separation, characterization, and purification of short RNA strands have been developed based on reversed-phase ion-pair liquid chromatography. The purification strategies were developed for both single… CONTINUE READING

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