Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells.

@article{Wang2011ReversedphaseCW,
  title={Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells.},
  author={Yuexi Wang and Feng Yang and Marina A. Gritsenko and Yingchun Wang and Therese R W Clauss and Tao Liu and Yufeng Shen and Matthew E. Monroe and Daniel Lopez-Ferrer and Theresa A. Reno and Ronald J. Moore and Richard L. Klemke and David G. Camp and Richard D. Smith},
  journal={Proteomics},
  year={2011},
  volume={11 10},
  pages={2019-26}
}
In this study, we evaluated a concatenated low pH (pH 3) and high pH (pH 10) reversed-phase liquid chromatography strategy as a first dimension for two-dimensional liquid chromatography tandem mass spectrometry ("shotgun") proteomic analysis of trypsin-digested human MCF10A cell sample. Compared with the more traditional strong cation exchange method, the… CONTINUE READING