Reversed-phase HPLC/FD method for the quantitative analysis of the neurotoxin BMAA (β-N-methylamino-L-alanine) in cyanobacteria.

@article{Cianca2012ReversedphaseHM,
  title={Reversed-phase HPLC/FD method for the quantitative analysis of the neurotoxin BMAA (β-N-methylamino-L-alanine) in cyanobacteria.},
  author={Rosa C. Cervantes Cianca and Maur{\'i}cio S Baptista and Lu{\'i}s Pinto Da Silva and Viviana R. Lopes and Vitor Vasconcelos},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={2012},
  volume={59 3},
  pages={379-84}
}
A method has been developed and optimized in order to detect and quantify the non-protein amino acid β-N-methylamino-L-alanine(BMAA) in cyanobacteria. The novelty of the method is that we have used methanol instead of acetonitrile as the eluent. The method includes extraction with 0.1 M trichloroacetic acid (free BMAA) or protein hydrolysis with 6 M hydrochloric acid (total BMAA), derivatization with AQC (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) and reversed-phase high-performance… CONTINUE READING