Reversed-phase C18 high-performance liquid chromatography of acidic and conjugated gibberellins

@inproceedings{Koshioka1983ReversedphaseCH,
  title={Reversed-phase C18 high-performance liquid chromatography of acidic and conjugated gibberellins},
  author={Masaji Koshioka and Jimpei Harada and Kiyotoshi Takeno and Masana Noma and Takeshi Sassa and Koichi Ogiyama and Joseph Sherwood Taylor and Stewart B Rood and Raymond L. Legge and Richard Persons Pharis},
  year={1983}
}
Abstract The retention times of gibberellins and their glucosyl esters and glucoside conjugates on C 18 reversed-phase high-performance liquid chromatographic (HPLC) columns were determined using gradient or isocratic elution with methanol—acetic acid 1% . The separation of double-bond gibberellin isomers was accomplished without the need for derivatization or the addition of salts. A combined HPLC—radiocounting with flow-through scintillation spectrometric procedure was suitable for the… CONTINUE READING

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