Reversed enantioselectivity of diisopropyl fluorophosphatase against organophosphorus nerve agents by rational design.

@article{Melzer2009ReversedEO,
  title={Reversed enantioselectivity of diisopropyl fluorophosphatase against organophosphorus nerve agents by rational design.},
  author={Marco Melzer and Julian C-H Chen and Anne Heidenreich and J{\"u}rgen G{\"a}b and Marianne Koller and Kai Kehe and M Blum},
  journal={Journal of the American Chemical Society},
  year={2009},
  volume={131 47},
  pages={17226-32}
}
Diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris is an efficient and robust biocatalyst for the hydrolysis of a range of highly toxic organophosphorus compounds including the nerve agents sarin, soman, and cyclosarin. In contrast to the substrate diisopropyl fluorophosphate (DFP) the nerve agents possess an asymmetric phosphorus atom, which leads to pairs of enantiomers that display markedly different toxicities. Wild-type DFPase prefers the less toxic stereoisomers of the substrates… CONTINUE READING

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