Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions.

@article{Vidal1996ReverseTA,
  title={Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions.},
  author={Marc Vidal and Rainer K. Brachmann and Ali Fattaey and E E Harlow and Jef D. Boeke},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 19},
  pages={10315-20}
}
Macromolecular interactions define many biological phenomena. Although genetic methods are available to identify novel protein-protein and DNA-protein interactions, no genetic system has thus far been described to identify molecules or mutations that dissociate known interactions. Herein, we describe genetic systems that detect such events in the yeast Saccharomyces cerevisiae. We have engineered yeast strains in which the interaction of two proteins expressed in the context of the two-hybrid… CONTINUE READING
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