Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA.

@article{Stangegaard2006ReverseTU,
  title={Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA.},
  author={Michael Stangegaard and Inge H\ogh Dufva and Martin Dufva},
  journal={BioTechniques},
  year={2006},
  volume={40 5},
  pages={
          649-57
        }
}
Reverse transcription of RNA is an invaluable method for gene expression analysis by real-time PCR or microarray methods. Random primers of varying lengths were compared with respect to their efficiency of priming reverse transcription reactions. The results showed that 15-nucleotide-long random oligonucleotides (pentadecamers) consistently yielded at least 2-fold as much cDNA as did random hexamers using either poly(A) RNA or an amplified version of messenger RNA (aRNA) as a template. The cDNA… CONTINUE READING
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