Reverse transcription slippage over the mRNA secondary structure of the LIP1 gene.

@article{Zhang2001ReverseTS,
  title={Reverse transcription slippage over the mRNA secondary structure of the LIP1 gene.},
  author={Yingpei Zhang and Hongyu Pan and Su Jun Gao},
  journal={BioTechniques},
  year={2001},
  volume={31 6},
  pages={1286, 1288, 1290, passim}
}
The secondary structures in mRNA often cause early termination during the synthesis of cDNA. In an attempt to determine the 5'-untranslated region (UTR) of the gene LIP1 using the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE), we found that reverse transcriptases skipped over the LIP1 RNA secondary structures and continued the DNA synthesis through RNA adapter sequences without early termination. A fragment of only three nucleotides upstream of the LIP1 translation initiation… CONTINUE READING