Reverse transcription polymerase chain reaction (RT-PCR) for diagnosis of respiratory syncytial virus infection in adults: use of a single-tube "hanging droplet" nested PCR.

Abstract

Rapid diagnosis of Respiratory Syncytial virus (RSV) infection is difficult in elderly persons due to the low quantities of virus shed. Therefore, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect viral RNA in respiratory secretions. A single-tube nested RT-PCR that used primers from a conserved F gene sequence was developed using… (More)

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Cite this paper

@article{Walsh2001ReverseTP, title={Reverse transcription polymerase chain reaction (RT-PCR) for diagnosis of respiratory syncytial virus infection in adults: use of a single-tube "hanging droplet" nested PCR.}, author={Edward E. Walsh and Ann R. Falsey and Ian A. Swinburne and Maria A. Formica}, journal={Journal of medical virology}, year={2001}, volume={63 3}, pages={259-63} }