Reverse transcription PCR-based sequence analysis of hepatitis C virus replicon RNA.

@article{Tellinghuisen2009ReverseTP,
  title={Reverse transcription PCR-based sequence analysis of hepatitis C virus replicon RNA.},
  author={Timothy L. Tellinghuisen and Brett Lindenbach},
  journal={Methods in molecular biology},
  year={2009},
  volume={510},
  pages={165-75}
}
Since the advent of efficient cell-culture methods for HCV replication and, more recently, infection, there has been a need to efficiently sequence the viral RNA in these systems. This need is especially urgent in light of the error-prone nature of HCV RNA replication, which leads to a variety of interesting mutations. The adaptation of hepatitis C… CONTINUE READING