Reverse-transcription PCR (RT-PCR).

@article{Bachman2013ReversetranscriptionP,
  title={Reverse-transcription PCR (RT-PCR).},
  author={Julia L. Bachman},
  journal={Methods in enzymology},
  year={2013},
  volume={530},
  pages={
          67-74
        }
}
RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g., see Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates and Circularized RT-PCR (cRT-PCR): analysis of RNA 5… CONTINUE READING
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