Reverse transcriptase from avian myeloblastosis virus.

  title={Reverse transcriptase from avian myeloblastosis virus.},
  author={G E Houts and M. Miyagi and C M Ellis and D F Beard and Joseph W. Beard},
  journal={Journal of virology},
  volume={29 2},
From lots of 20 to 30 g of avian myeloblastosis virus RNA-dependent DNA polymerase was obtained in preparations of purity greater than 95% by using a two-step column chromatographic procedure employing DEAE (DE 52) and carboxymethylcellulose (CM 52.). Yields of RNA-dependent DNA polymerase varied from approximately 20,000 to 35,000 U/g of virus. Specific activity of the enzyme was about 35,000 to 60,000 U/mg of protein. Free of detectable RNase activity, the product exhibited a molecular weight… CONTINUE READING

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