Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5'-RACE and primer extension.

@article{Chen2001ReverseTA,
  title={Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5'-RACE and primer extension.},
  author={Donna T Chen and John T Patton},
  journal={BioTechniques},
  year={2001},
  volume={30 3},
  pages={
          574-80, 582
        }
}
In determining the terminal sequences of the genomic dsRNAs of rotavirus by 5'-rapid amplification of cDNA ends (5'-RACE), it was found that most of the viral cDNAs contained extra nucleotides at their 5' termini that had not been reported before on any rotavirus sequence. Although the extra nucleotides could be dA, dC, dG, or dT residues, the extra nucleotides on the cDNAs usually consisted of a single dT residue. Experiments performed with DNA/RNA duplexes indicated that reverse transcriptase… CONTINUE READING
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Rotaviruses and their replication , p . 731 - 776

  • M. K. Estes
  • 1996

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