Reverse transcriptase activity and Ty RNA are associated with virus-like particles in yeast

@article{Mellor1985ReverseTA,
  title={Reverse transcriptase activity and Ty RNA are associated with virus-like particles in yeast},
  author={Jane Mellor and Michael H. Malim and Keith Gull and Mick F. Tuite and Shirley J. McCready and Teresa P. Dibbayawan and Susan Mary Kingsman and Alan John Kingsman},
  journal={Nature},
  year={1985},
  volume={318},
  pages={583-586}
}
The Ty element of yeast represents a class of eukaryotic transposons that show remarkable structural similarity to retroviral proviruses1,2. Recently, these comparisons have been strengthened by a series of observations on the yeast Ty element: (1) Ty transposes via an RNA intermediate3; (2) it contains a sequence (Fig. 1) which, when translated, is homologous to a conserved region found in all reverse transcriptases4,5; (3) a fusion protein encoded by Ty is produced by a frameshift event that… Expand
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TLDR
Functional properties of putative proteins encoded by the yeast transposable element, Ty1, by overexpression of TY genes support the hypothesis that TYB is expressed as a TYA/TYB fusion protein which is processed by a TYB-encoded protease activity. Expand
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The Ty 1 LTR-Retrotransposon of Budding Yeast , Saccharomyces cerevisiae
Long-terminal repeat (LTR)-retrotransposons generate a copy of their DNA (cDNA) by reverse transcription of their RNA genome in cytoplasmic nucleocapsids. They are widespread in the eukaryoticExpand
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TLDR
The results support the idea that Ty elements and retroviruses share a common origin and the numerous virus-like particles visible in thin sections of Ty transposition-induced cells appear to be infectious. Expand
A retrovirus-like strategy for expression of a fusion protein encoded by yeast transposon Ty1
TLDR
It is shown that the yeast transposon produces a fusion protein by a specific frameshifting event that fuses two out-of-phase open reading frames (ORFs) and is remarkably similar to that used by retroviruses such as Rous sarcoma virus to produce Pr180gag–pol. Expand
Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression.
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TLDR
The DNA sequence of the transposable element Ty912 of yeast is determined and it is shown that synthesis of this fusion protein probably does not occur by RNA splicing and is consistent with a mechanism of translational frameshifting occurring within the region of overlap between the 3' end of tya912 and the 5'End of tyb912. Expand
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TLDR
The nucleotide sequence of a complete yeast Ty element (Ty-pY109) which is located near a tRNA(Lys1) gene is determined and the organisation of the coding sequences in Ty resembles that found in retroviral proviruses and the copia-like elements in Drosophila. Expand
Ty elements transpose through an RNA intermediate
TLDR
It is demonstrated that during transposition, Ty sequence information flows from DNA to RNA to DNA, and the pattern of sequence inheritance in progeny Ty insertions derived from the marked Ty follows the predictions of the model of retroviral reverse transcription. Expand
RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.
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The RNA homologous to the yeast transposable element Ty1 is one of the more abundant poly(A)+ RNAs in many strains of the yeast Saccharomyces cerevisiae. The 5' and 3' ends of Ty1 RNA have beenExpand
Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.
TLDR
Observations strongly suggest that the p1 proteins of Ty elements are functionally significant and that they have been subject to selection. Expand
A mutant murine leukemia virus with a single missense codon in pol is defective in a function affecting integration.
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TLDR
It is concluded that the MuLV-SF1 pol gene is defective for a function that is required for normal integrative recombination and dissociable from DNA synthesis. Expand
The identification and high level expression of a protein encoded by the yeast Ty element.
TLDR
Data show for the first time that Ty elements encode proteins and illustrate the general usefulness of high efficiency expression vectors for the detection of rare products. Expand
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TLDR
The 3' end of the pol gene serves as an "int" locus and encodes a protein mediating integration of retrovirus DNA through interaction with att, showing that the att- virus provided a transacting function allowing integration of viral DNA derived from the mutant bearing a wild-type att site. Expand
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