Reverse sample genome probing, a new technique for identification of bacteria in environmental samples by DNA hybridization, and its application to the identification of sulfate-reducing bacteria in oil field samples.

@article{Voordouw1991ReverseSG,
  title={Reverse sample genome probing, a new technique for identification of bacteria in environmental samples by DNA hybridization, and its application to the identification of sulfate-reducing bacteria in oil field samples.},
  author={Gerrit Voordouw and Johanna K. Voordouw and Roxann R. Karkhoff-Schweizer and Phillip M. Fedorak and Donald William Speck Westlake},
  journal={Applied and environmental microbiology},
  year={1991},
  volume={57 11},
  pages={
          3070-8
        }
}
A novel method for the identification of bacteria in environmental samples by DNA hybridization is presented. It is based on the fact that, even within a genus, the genomes of different bacteria may have little overall sequence homology. This allows the use of the labeled genomic DNA of a given bacterium (referred to as a "standard") to probe for its presence and that of bacteria with highly homologous genomes in total DNA obtained from an environmental sample. Alternatively, total DNA… CONTINUE READING

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